Instituto de Salud Carlos III
Vectores de enfermedades de interés en salud pública: flebotominos, anofelinos y triatominos, biología, competencia vectorial, colonias de laboratorio, infecciones experimentales, ciclo intravectorial, sondeos entomológicos, poder infectante de reservorios potenciales de leishmaniasis, filogenia molecular.
- Insecticidas y repelentes: evaluación de eficacia y estudio de resistencias.
- Ensayos de vacunas frente a leishmaniasis: desafío, infectividad. Caracterización molecular de la saliva de P. perniciosus y P. argentipes. Genómica, proteómica, marcadores de exposición a la picadura, factores de virulencia, vacunas.
- Cambio global y su impacto sobre la dispersión de la leishmaniasis: mapas de riesgo, modelos predictivos. - Vigilancia de dípteros exóticos importados: culícidos, Aedes albopictus, Aedes aegypti, Anopheles sp., aeropuertos y puertos.
- Xenodiagnóstico de Leishmaniasis y Enfermedad de Chagas. Detección molecular de Leishmania infantum en flebotomos.
- Identificación molecular del origen de la sangre ingerida por vectores.
Identificación de artrópodos de interés médico: vectores, ectoparásitos, endoparásitos, miasis, plagas. La Unidad forma parte del grupo colaborador de la OMS para Leishmaniasis, es miembro de la red europea VBORNET y es laboratorio integrante del Observatorio de Salud y Cambio Climático.
Publications
Influence of the Microenvironment in the Transcriptome of Leishmania infantum Promastigotes: Sand Fly versus Culture, Paul Andrew Bates, Pedro J. Alcolea, Ana Alonso, Mercedes Domínguez, Víctor Parro, Maribel Jiménez, Ricardo Molina, Vicente Larraga. PLOS Neglected Tropical Diseases. 2016
In vitro infectivity and differential gene expression of Leishmania infantum metacyclic promastigotes: negative selection with peanut agglutinin in culture versus isolation from the stomodeal valve of Phlebotomus perniciosus, Pedro J. Alcolea, Ana Alonso, María A. Degayón, Mercedes Moreno-Paz, Maribel Jiménez, Ricardo Molina, Vicente Larraga. BMC Genomics. 2016
Phleboviruses detection in Phlebotomus perniciosus from a human leishmaniasis focus in South-West Madrid region, Spain, Maria Elena Remoli, Maribel Jiménez, Claudia Fortuna, Eleonora Benedetti, Antonella Marchi, Domenico Genovese, Marina Gramiccia, Ricardo Molina, Maria Grazia Ciufolini. Parasites & Vectors. 2016
Natural transmission of Leishmania infantum through experimentally infected Phlebotomus perniciosus highlights the virulence of Leishmania parasites circulating in the human visceral leishmaniasis outbreak in Madrid, Spain, Inés Martín-Martín, Maribel Jiménez, Estela González, César Eguiluz, Ricardo Molina. Veterinary Research. 2015
Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene, Estela González, Montserrat Gállego, Ricardo Molina, Alba Abras, M. Magdalena Alcover, Cristina Ballart, Anna Fernández, Maribel Jiménez. Acta Tropica. 2015
Sampling strategies for phlebotomine sand flies (Diptera: Psychodidae) in Europe, B. Alten, Y. Ozbel, K. Ergunay, O.E. Kasap, B. Cull, M. Antoniou, E. Velo, J. Prudhomme, R. Molina, A.-L. Bañuls, F. Schaffner, G. Hendrickx, W. Van Bortel, J.M. Medlock. Bulletin of Entomological Research. 2015
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits, Sima Rafati, Inés Martín-Martín, Ricardo Molina, Maribel Jiménez. PLOS ONE. 2015
New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area, Jorian Prudhomme, Céline Toty, Ozge Erisoz Kasap, Nil Rahola, Baptiste Vergnes, Carla Maia, Lenea Campino, Maria Antoniou, Maribel Jimenez, Ricardo Molina, Arnaud Cannet, Bulent Alten, Denis Sereno, Anne-Laure Bañuls. Acta Tropica. 2015
First Evidence of Intraclonal Genetic Exchange in Trypanosomatids Using Two Leishmania infantum Fluorescent Transgenic Clones, Paul Andrew Bates, Estefanía Calvo-Álvarez, Raquel Álvarez-Velilla, Maribel Jiménez, Ricardo Molina, Yolanda Pérez-Pertejo, Rafael Balaña-Fouce, Rosa M. Reguera. PLoS Neglected Tropical Diseases. 2014
Could wild rabbits (Oryctolagus cuniculus) be reservoirs for Leishmania infantum in the focus of Madrid, Spain?, Maribel Jiménez, Estela González, Inés Martín-Martín, Sonia Hernández, Ricardo Molina. Veterinary Parasitology. 2014
High levels of anti-Phlebotomus perniciosus saliva antibodies in different vertebrate hosts from the re-emerging leishmaniosis focus in Madrid, Spain, Inés Martín-Martín, Ricardo Molina, Iva Rohoušová, Jan Drahota, Petr Volf, Maribel Jiménez. Veterinary Parasitology. 2014
Recombinant Antigens from Phlebotomus perniciosus Saliva as Markers of Canine Exposure to Visceral Leishmaniases Vector, Shaden Kamhawi, Jan Drahota, Ines Martin-Martin, Petra Sumova, Iva Rohousova, Maribel Jimenez, Ricardo Molina, Petr Volf. PLoS Neglected Tropical Diseases. 2014
Characterisation of the ex vivo virulence of Leishmania infantum isolates from Phlebotomus perniciosus from an outbreak of human leishmaniosis in Madrid, Spain, Gustavo Domínguez-Bernal, Maribel Jiménez, Ricardo Molina, Lara Ordóñez-Gutiérrez, Abel Martínez-Rodrigo, Alicia Mas, Maria Teresa Cutuli, Javier Carrión. Parasites & Vectors. 2014
Identifying salivary antigens of Phlebotomus argentipes by a 2DE approach, Inés Martín-Martín, Ricardo Molina, Maribel Jiménez. Acta Tropica. 2013
Control of multiple arthropod vector infestations with subolesin/akirin vaccines, Juan A. Moreno-Cid, José M. Pérez de la Lastra, Margarita Villar, Maribel Jiménez, Rocío Pinal, Agustín Estrada-Peña, Ricardo Molina, Javier Lucientes, Christian Gortázar, José de la Fuente. Vaccine. 2013
Predicting the risk of an endemic focus of Leishmania tropica becoming established in south-western Europe through the presence of its main vector, Phlebotomus sergenti Parrot, 1917, S. D. BARÓN, F. MORILLAS-MÁRQUEZ, M. MORALES-YUSTE, V. DÍAZ-SÁEZ, M. GÁLLEGO, R. MOLINA, J. MARTÍN-SÁNCHEZ. Parasitology. 2013
Endemic Transmission of Visceral Leishmaniasis in Bhutan, T. Yangzom, I. Cruz, C. Bern, D. Argaw, M. den Boer, I. D. Velez, S. K. Bhattacharya, R. Molina, J. Alvar. American Journal of Tropical Medicine and Hygiene. 2012
The hare (Lepus granatensis) as potential sylvatic reservoir of Leishmania infantum in Spain, R. Molina, M.I. Jiménez, I. Cruz, A. Iriso, I. Martín-Martín, O. Sevillano, S. Melero, J. Bernal. Veterinary Parasitology. 2012
Efficacy of 65% permethrin applied to dogs as a spot-on against Phlebotomus perniciosus, R. Molina, C. Espinosa-Góngora, R. Gálvez, A. Montoya, M.A. Descalzo, M.I. Jiménez, D. Dado, G. Miró. Veterinary Parasitology. 2012
An insight into the Phlebotomus perniciosus saliva by a proteomic approach, Inés Martín-Martín, Ricardo Molina, Maribel Jiménez. Acta Tropica. 2012
Questionnaire-based survey on the clinical management of canine leishmaniosis in the Madrid region (central Spain), R. Gálvez, G. Miró, M.A. Descalzo, R. Molina. Preventive Veterinary Medicine. 2011
Mapping the Current Distribution and Predicted Spread of the Leishmaniosis Sand Fly Vector in the Madrid Region (Spain) Based on Environmental Variables and Expected Climate Change, Rosa Gálvez, Miguel A. Descalzo, Irene Guerrero, Guadalupe Miró, Ricardo Molina. Vector-Borne and Zoonotic Diseases. 2011
Infectivity to Phlebotomus perniciosus of dogs naturally parasitized with Leishmania infantum after different treatments, Guadalupe Miró, Rosa Gálvez, Cristeta Fraile, Miguel A Descalzo, Ricardo Molina. Parasites & Vectors. 2011
Predicting the distribution of canine leishmaniasis in western Europe based on environmental variables, ANA O. FRANCO, CLIVE R. DAVIES, ADRIAN MYLNE, JEAN-PIERRE DEDET, MONTSERRAT GÁLLEGO, CRISTINA BALLART, MARINA GRAMICCIA, LUIGI GRADONI, RICARDO MOLINA, ROSA GÁLVEZ, FRANCISCO MORILLAS-MÁRQUEZ, SERGIO BARÓN-LÓPEZ, CARLOS ALVES PIRES, MARIA ODETE AFONSO, PAUL D. READY, JONATHAN COX. Parasitology. 2011
Characterization of Aedes albopictus akirin for the control of mosquito and sand fly infestations, Juan A. Moreno-Cid, Maribel Jiménez, Sylvie Cornelie, Ricardo Molina, Pedro Alarcón, Marie-Noelle Lacroix, Rocío Pinal, Sarah Delacour, Javier Lucientes, Mario Canales, José M. Pérez de la Lastra, Margarita Villar, José de la Fuente. Vaccine. 2010
Genetic structure of Phlebotomus (Larroussius) ariasi populations, the vector of Leishmania infantum in the western Mediterranean: Epidemiological implications, Fernando A.L. Franco, Francisco Morillas-Márquez, Sergio D. Barón, Manuel Morales-Yuste, Rosa Gálvez, Victoriano Díaz, Bernard Pesson, Carlos Alves-Pires, Jerome Depaquit, Ricardo Molina, M. Odette Afonso, Montserrat Gállego, Souad Guernaoui, Azzedine Bounamous, Joaquina Martín-Sánchez. International Journal for Parasitology. 2010
Seasonal trends and spatial relations between environmental/meteorological factors and leishmaniosis sand fly vector abundances in Central Spain, R. Gálvez, M.A. Descalzo, G. Miró, M.I. Jiménez, O. Martín, F. Dos Santos-Brandao, I. Guerrero, E. Cubero, R. Molina. Acta Tropica. 2010
Emerging trends in the seroprevalence of canine leishmaniosis in the Madrid region (central Spain), R. Gálvez, G. Miró, M.A. Descalzo, J. Nieto, D. Dado, O. Martín, E. Cubero, R. Molina. Veterinary Parasitology. 2010
Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin, Mario Canales, Victoria Naranjo, Consuelo Almazán, Ricardo Molina, Suzana A. Tsuruta, Matias P. J. Szabó, Raúl Manzano-Roman, José M. Pérez de la Lastra, Katherine M. Kocan, María Isabel Jiménez, Javier Lucientes, Margarita Villar, José de la Fuente. Parasitology Research. 2009
Comparative real-time kinetic analysis of human complement killing of Leishmania infantum promastigotes derived from axenic culture or from Phlebotomus perniciosus, Inmaculada Moreno, Ricardo Molina, Alfredo Toraño, Edurne Laurin, Esther García, Mercedes Domínguez. Microbes and Infection. 2007
A leishmaniosis surveillance system among stray dogs in the region of Madrid: ten years of serodiagnosis (1996–2006), G. Miró, A. Montoya, M. Mateo, A. Alonso, S. García, A. García, M. J. Caballero, R. Molina. Parasitology Research. 2007
Evaluation of the efficacy of a topically administered combination of imidacloprid and permethrin against Phlebotomus perniciosus in dog, Guadalupe Miró, Rosa Gálvez, Marta Mateo, Ana Montoya, Miguel Angel Descalzo, Ricardo Molina. Veterinary Parasitology. 2007
Canine Leishmaniasis, Jorge Alvar, Carmen Cañavate, Ricardo Molina, Javier Moreno, Javier Nieto. . 2004
Intradermal Infection Model for Pathogenesis and Vaccine Studies of Murine Visceral Leishmaniasis, S. Ahmed, M. Colmenares, L. Soong, K. Goldsmith-Pestana, L. Munstermann, R. Molina, D. McMahon-Pratt. Infection and Immunity. 2003
Experimental infection of Phlebotomusperniciosus and determination of the natural infection rates of Leishmaniainfantum in dogs, José L Guarga, J Lucientes, Miguel A Peribáñez, Ricardo Molina, Marı́a J Gracia, Juan A Castillo. Acta Tropica. 2000
Variability of Leishmania (Leishmania) infantum among stocks from immunocompromised, immunocompetent patients and dogs in Spain, M Jiménez. FEMS Microbiology Letters. 1995
A laboratory model of canine leishmaniasis: the inoculation of dogs withLeishmania infantumpromastigotes from midguts of experimentally infected phlebotomine sandflies, R. Killick-Kendrick, M. Killick-Kendrick, E. Pinelli, G. Del Real, R. Molina, M.M. Vitutia, M.C. Cañavate, J. Nieto. Parasite. 1994
Infectivity of dogs naturally infected with Leishmania infantum to colonized Phlebotomus perniciosus, R. Molina, C. Amela, J. Nieto, M. San-Andrés, F. González, J.A. Castillo, J. Lucientes, J. Alvar. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1994
Prevalence of Leishmania infection among AIDS patients, J Alvar, B Gutierrez-Solar, R Molina, R Lopez-Velez, A Garcia-Camacho, P Martinez, F Laguna, E Cercenado, A Galmes. The Lancet. 1992
Isolation of Leishmania infantum from the blood of a patient with AIDS using sandflies, R. Molina, R. López-Vélez, B. Gutiérrez-Solar, M.I. Jiménez, J. Alvar. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1992
